Steroid isolation , depending on context, is the isolation of chemical matter required for chemical structure elucidation, derivitzation or degradation chemistry, biological testing, and other research needs (generally milligrams to grams, but often more  or the isolation of "analytical quantities" of the substance of interest (where the focus is on identifying and quantifying the substance (for example, in biological tissue or fluid). The amount isolated depends on the analytical method, but is generally less than one microgram.  [ page needed ] The methods of isolation to achieve the two scales of product are distinct, but include extraction , precipitation, adsorption , chromatography , and crystallization . In both cases, the isolated substance is purified to chemical homogeneity; combined separation and analytical methods, such as LC-MS , are chosen to be "orthogonal"—achieving their separations based on distinct modes of interaction between substance and isolating matrix—to detect a single species in the pure sample. Structure determination refers to the methods to determine the chemical structure of an isolated pure steroid, using an evolving array of chemical and physical methods which have included NMR and small-molecule crystallography .  : 10–19 Methods of analysis overlap both of the above areas, emphasizing analytical methods to determining if a steroid is present in a mixture and determining its quantity. 
Melanin (skin pigmentation) has also been reported to have its production reduced secondary to reduces production of the enzyme tyrosinase , which catalyzes the rate-limiting step in melanin synthesis.  This study noted that in B16F1 melanoma cells that incubation with up to 200ug/mL failed to exert cytotoxic effects and reduced melanin content at 25ug/mL (to 67% of control) and more-so at 50ug/mL and 100ug/mL reducing melanin down to 40% of control cells (with no significant difference between these two concentrations).  mRNA of tyrosinase was potently reduced to +/-% of control with 25ug/mL with higher doses being insignificantly better.